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KMID : 0378019800230100105
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New Medical Journal
1980 Volume.23 No. 10 p.105 ~ p.110
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Repetitive Sequences in the DNAs of Mouse Tumor Cells (Ⅱ)
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金昇元/Kimm, Seung Won
鄭弘根/박주배/徐延瑄/Chung, Hong Keun/Park, Joo Bae/Seo, Jeong Sun
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Abstract
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This study was carried out to examine the complete extent of the reassociation kinetics of the DNA of mouse tumor cells. The transplantable tumors used in the experiment were Sarcoma 180, and Yun Liposarcoma. Before the reassociation experiment the purity of DNA preparations was confirmed by its melting behavior (temperature denaturation).
The results were analyzed by comparing the pooled DNA of normal liver cells with that of tumor cells.
The following results were obtained.
1. The amount of non-repetitive fraction of each tumor cell DNA are as follows:
Sarcoma 180 57.0% (Per total genome) Yun liposarcoma 52.0% normal liver cell 68.5%
2. The amount of highly repetitive fraction of tumor cell DNA´s is 15-16% of the total genome and repetition frequencies are L200,000-1,600,000.
3. In the case of the intermediate fraction, its amount in the tumor cell is approximately twice that of normal cell: (Sarcoma i80 DNA, 21.0% : Yun liposarcoma DNA, 24.0% normal liver cell DNA : 11.5% of total genome).
The repetition requencies of intermediate fraction of tumor cell DNA increase by 10 times, compared with that of normal liver cell DNA.
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KEYWORD
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